Description |
Timeframe |
| Cloning & mapping of locus |
1 Month |
| Engineering a gene targeting construct |
1 Month |
| ¡°Test¡± microinjection |
1 week |
| If DNA kills embryos: |
DNA must be remade by lab requesting microinjection or by
us |
| If DNA is of good quality: |
|
| Microinjection |
1 week |
| Birth of potential founders |
3 weeks |
| Identification of potential founders by coat color |
1.5 - 2 weeks |
| Weaning and tagging of chimeras |
~ 3-4 weeks from birth (depends on size of pups) |
| Begin breeding chimeras to check germline transmission |
~3 weeks (mice are 6 weeks old and sexually mature) |
| Birth of F1 pups |
~ 3 weeks |
| Weaning, tagging and tailing pups |
~ 3-4 weeks (depends on size of pups) |
| DNA isolation from tails (Laird¡¯s method) |
2 days |
| Notification DNA is ready for pickup |
Immediately |
| Starvax Service begins paperwork for transfer of animals
to investigator¡¯s lab |
Immediately |
| Mice are transferred |
Dependent on client location where mice are shipped |
When your lab has verified the sequence of your
transgenic construct you will either give us a bacterial stock
or agar plate OR give us the purified DNA (2XCsCl preps):
